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验证数据展示

  • Single Cell Sequencing experiment G65169-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65169-1-5C on Resting PBMC

    G65169-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65169-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65169-1-5C on PBMC treated with 4hr LPS + TI

    G65169-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human CD163 (GHI/61)

CD163 Monoclonal Antibody for Single Cell (Intra)
Cat No. G65169-1-5C

产品说明书

CloneNo. GHI/61

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

Single Cell (Intra)

别名

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

缓冲液配方:  PBS, Azide
PBS, Azide
偶联物:  5CFLX
规格价格库存

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65169-1-5C targets CD163 in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原

Glycoprotein preparation from hairy cell leukemia spleen

种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD163 (GHI/61)
别名 CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163
计算分子量 1156 aa, 125 kDa
GenBank蛋白编号BC051281
基因名称 CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
RRIDAB_3673925
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGACCGCGACCTGATAACCCATATAAGAAA
条形码序列ACCGCGACCTGATAA
形式Liquid
UNIPROT IDQ86VB7
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3.
储存条件2-8°C Stable for one year after shipment.

背景介绍

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol
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