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验证数据展示

  • Single Cell Sequencing experiment G65056-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65056-1-5C on Resting PBMC

    G65056-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65056-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65056-1-5C on PBMC treated with 4hr LPS + TI

    G65056-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

MultiPro® 5CFLX Anti-Human CD14 (UCHM-1)

CD14 Monoclonal Antibody for Single Cell
Cat No. G65056-1-5C

产品说明书

CloneNo. UCHM-1

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

Single Cell

别名

CD14, CD14 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65056-1-5C targets CD14 in Single Cell applications and shows reactivity with Human samples.

经测试应用 Single Cell Application Description
经测试反应性 Human
免疫原 Thymocytes and peripheral blood lymphocytes from a remission AML patient 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD14 (UCHM-1)
别名 CD14, CD14 molecule
计算分子量 375 aa, 40 kDa
GenBank蛋白编号BC010507
基因名称 CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
RRIDAB_3673906
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGCTTGCGTCTACCGCCCCCATATAAGAAA
条形码序列CTTGCGTCTACCGCC
形式 Liquid
UNIPROT IDP08571
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol