验证数据展示
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G17626-1-5C targets CD127/IL-7R in Single Cell (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag11844 Product name: Recombinant human IL-7R protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 266-459 aa of BC067537 Sequence: KRIKPIVWPSLPDHKKTLEHLCKKPRKNLNVSFNPESFLDCQIHRVDDIQARDEVEGFLQDTFPQQLEESEKQRLGGDVQSPNCPSEDVVITPESFGRDSSLTCLAGNVSACDAPILSPSRSLDCRESGKNGPHVYQDLLLSLGTTNSTLPPPFSLQSGILTLNPVAQGQPILTSLGSNQEEAYVTMSSFYQNQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Polyclonal |
| 全称 | MultiPro® 5CFLX Anti-Human CD127/IL-7R (Polyclonal) |
| 别名 | CD127, CDW127, IL 7 receptor subunit alpha, IL 7R alpha, IL 7R subunit alpha, IL 7RA, IL7R, IL-7R, IL7RA, ILRA, interleukin 7 receptor |
| 计算分子量 | 459 aa, 52 kDa |
| GenBank蛋白编号 | BC067537 |
| 基因名称 | CD127 |
| Gene ID (NCBI) | 3575 |
| ENSEMBL Gene ID | ENSG00000168685 |
| RRID | AB_3673892 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGCGGCTCGGACCATAGCCCATATAAGAAA |
| 条形码序列 | CGGCTCGGACCATAG |
| 形式 | Liquid |
| UNIPROT ID | P16871 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3. |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).
Protocols
| Our current MultiPro staining protocol for the cocktail | Download protocol |
| 10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |
