验证数据展示
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
| Positive Single Cell detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| SINGLE CELL | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G65086-1-5C targets CD11c in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra), Single Cell Application Description |
| 经测试反应性 | Human |
| 免疫原 |
Rheumatoid synovial cells and human monocytes 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Monoclonal |
| 全称 | MultiPro® 5CFLX Anti-Human CD11c (3.9) |
| 别名 | CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6 |
| 计算分子量 | 1169 aa, 129 kDa |
| GenBank蛋白编号 | BC038237 |
| 基因名称 | CD11c |
| Gene ID (NCBI) | 3687 |
| ENSEMBL Gene ID | ENSG00000140678 |
| RRID | AB_3673910 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGAAGCAATACTTATACCCCATATAAGAAA |
| 条形码序列 | AAGCAATACTTATAC |
| 形式 | Liquid |
| UNIPROT ID | P20702 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3. |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).
Protocols
| Our current MultiPro staining protocol for the cocktail | Download protocol |
| 10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol |
