Speedy™ Mouse CD31 One-Step ELISA Kit
Sensitivity
4.2 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse CD31 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 4.2 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 18613 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 103% | 100%-109% |
| Lysate | 112% | 99%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,610.8 | 67.5 | 2.6 |
| 2 | 8 | 672.2 | 17.2 | 2.6 |
| 3 | 8 | 340.1 | 9.0 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,576.4 | 75.6 | 2.9 |
| 2 | 16 | 669.5 | 18.8 | 2.8 |
| 3 | 16 | 341.0 | 11.6 | 3.4 |
Background Information
CD31, also known as platelet endothelial cell adhesion molecule1 (PECAM-1), is a 130 kDa platelet-endothelial cell (EC) adhesion molecule initially identified from ECs and platelets and later from blood leucocytes. CD31 was initially classified as a cell adhesion molecule, later studies suggested that CD31 triggers downstream inhibitory signaling upon trans-homophilic CD31 engagement during cell-cell interaction. CD31 signaling participates in regulating leucocyte detachment, T-cell activation, platelet activation, and angiogenesis, all of which are critical to the pathogenesis of atherosclerosis and abdominal aortic aneurysms (AAAs).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Pecam, PECAM 1, Pecam1, PECAM-1 |
FAQ
1、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
