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验证数据展示

  • WB analysis using 25257-1-AP

    WB analysis using 25257-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25257-1-AP (MYBPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skeletal muscle using 25257-1-AP

    IP experiment of mouse skeletal muscle using 25257-1-AP

    IP result of anti-MYBPC2 (IP:25257-1-AP, 4ug; Detection:25257-1-AP 1:40000) with mouse skeletal muscle tissue lysate 1040 ug.

  • IF Staining of mouse skeletal muscle using 25257-1-AP

    IF Staining of mouse skeletal muscle using 25257-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYBPC2 antibody (25257-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MYBPC2 Polyclonal antibody

MYBPC2 Polyclonal Antibody for WB, IF-P, IP, ELISA
Cat No. 25257-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, IP, ELISA

别名

MYBPCF, Fast MyBP C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IF-P detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25257-1-AP targets MYBPC2 in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MYBPC2 fusion protein Ag19424 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin binding protein C, fast type
别名 MYBPCF, Fast MyBP C
计算分子量 1141 aa, 128 kDa
观测分子量 130 kDa
GenBank蛋白编号BC136389
基因名称 MYBPC2
Gene ID (NCBI) 4606
RRIDAB_3669466
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14324
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYBPC2, also known as MYBPCF, fMyBP-C, is a member of the myosin-binding protein C (MYBPC)family. MYBPC is a myosin-associated protein found in the cross-bridge-bearing zone, or C region, of A bands in striated muscle. MYBPC has three paralogs encoded by unique genes, including slow skeletal (MYBPC1), fast skeletal (MYBPC2), and cardiac (MYBPC3). Slow skeletal MyBP-C (sMyBP-C), fast skeletal MyBP-C (fMyBP-C), and cardiac MyBP-C (cMyBP-C) proteins are highly conserved with over 90% homology. They play unique muscle-specific structural and regulatory roles in actomyosin interactions and contractility in striated muscles, including both cardiac and skeletal muscles (PMID: 35832193, 23936073).

实验方案

Product Specific Protocols
WB protocol for MYBPC2 antibody 25257-1-APDownload protocol
IF protocol for MYBPC2 antibody 25257-1-APDownload protocol
IP protocol for MYBPC2 antibody 25257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols