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验证数据展示

  • IHC staining of mouse small intestine using 23640-1-AP

    IHC staining of mouse small intestine using 23640-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 23640-1-AP (MRGPRD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat dorsal root ganglion using 23640-1-AP

    IHC staining of rat dorsal root ganglion using 23640-1-AP

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 23640-1-AP (MRGPRD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 23640-1-AP

    IF Staining of mouse small intestine using 23640-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using MRGPRD antibody (23640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 23640-1-AP

    IF Staining of mouse small intestine using 23640-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using MRGPRD antibody (23640-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRGPRD Polyclonal antibody

MRGPRD Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 23640-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, ELISA

别名

Beta alanine receptor, Beta-alanine receptor, G-protein coupled receptor TGR7, MAS related GPR, member D, Mas-related G-protein coupled receptor member D

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse small intestine tissue, rat dorsal root ganglion tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23640-1-AP targets MRGPRD in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20316

Product name: Recombinant human MRGPRD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 230-321 aa of BC156660

Sequence: FLICSLPLSIYWFVLYWLSLPPEMQVLCFSLSRLSSSVSSSANPVIYFLVGSRRSHRLPTRSLGTVLQQALREEPELEGGETPTVGTNEMGA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MAS-related GPR, member D
别名 Beta alanine receptor, Beta-alanine receptor, G-protein coupled receptor TGR7, MAS related GPR, member D, Mas-related G-protein coupled receptor member D
计算分子量 321 aa, 36 kDa
GenBank蛋白编号BC156660
基因名称 MRGPRD
Gene ID (NCBI) 116512
RRIDAB_3669414
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ8TDS7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRGPRD (MAS-related G-protein coupled receptor, D), also referred to as hGPCR45 or TGR7, was identified as a novel GPCR in murine and human genomes. As for MRGD, its expression was found in dorsal root ganglia (DRG) and co-localized with Vanilloid receptor-1 (VR-1), which is an essential receptor for heat and pain sensation (PMID: 22715397).

实验方案

Product Specific Protocols
IF protocol for MRGPRD antibody 23640-1-APDownload protocol
IHC protocol for MRGPRD antibody 23640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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