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MRGPRD Polyclonal antibody

MRGPRD Polyclonal Antibody for IF-P, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, IHC, ELISA

Conjugate

Unconjugated

Cat No : 23640-1-AP

Print datasheet

Synonyms

Beta alanine receptor, MAS related GPR, member D, MRGD, MRGPRD, TGR7

验证数据展示

  • IHC staining of mouse small intestine using 23640-1-AP

    IHC staining of mouse small intestine using 23640-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 23640-1-AP (MRGPRD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 23640-1-AP

    IF Staining of mouse small intestine using 23640-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using MRGPRD antibody (23640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat dorsal root ganglion using 23640-1-AP

    IHC staining of rat dorsal root ganglion using 23640-1-AP

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 23640-1-AP (MRGPRD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 23640-1-AP

    IF Staining of mouse small intestine using 23640-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using MRGPRD antibody (23640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse small intestine tissue, rat dorsal root ganglion tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse small intestine tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23640-1-AP targets MRGPRD in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, IHC, ELISA
Tested Reactivity human, mouse, rat
Immunogen MRGPRD fusion protein Ag20316 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MAS-related GPR, member D
Synonyms Beta alanine receptor, MAS related GPR, member D, MRGD, MRGPRD, TGR7
Calculated Molecular Weight 321 aa, 36 kDa
GenBank Accession NumberBC156660
Gene Symbol MRGPRD
Gene ID (NCBI) 116512
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8TDS7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRGPRD (MAS-related G-protein coupled receptor, D), also referred to as hGPCR45 or TGR7, was identified as a novel GPCR in murine and human genomes. As for MRGD, its expression was found in dorsal root ganglia (DRG) and co-localized with Vanilloid receptor-1 (VR-1), which is an essential receptor for heat and pain sensation (PMID: 22715397).

实验方案

Product Specific Protocols
IHC protocol for MRGPRD antibody 23640-1-APDownload protocol
IF protocol for MRGPRD antibody 23640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols