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验证数据展示

  • WB analysis using 67985-1-Ig

    WB analysis using 67985-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67985-1-Ig (MON1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of A549 using 67985-1-Ig

    WB analysis of A549 using 67985-1-Ig

    WB result of MON1B antibody (67985-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MON1B transfected A549 cells.

  • IF Staining of HeLa using 67985-1-Ig

    IF Staining of HeLa using 67985-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MON1B antibody (67985-1-Ig, Clone: 3E7C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  • KD/KO验证

MON1B Monoclonal antibody

MON1B Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67985-1-Ig

产品说明书

CloneNo. 3E7C6

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

HSRG1, KIAA0872, MON1 homolog B (yeast), MON1B, SAND2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, HepG2 cells, K-562 cells, SW480 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67985-1-Ig targets MON1B in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag9920

Product name: Recombinant human MON1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 199-547 aa of BC024277

Sequence: STLTRASVARIFAHKQNYDLRRLLAGSERTLDRLLDSMEQDPGALLLGAVRCVPLARPLRDALGALLRRCTAPGLALSVLAVGGRLITAAQERNVLAECRLDPADLQLLLDWVGAPAFAAGEAWAPVCLPRFNPDGFFYAYVARLDAMPVCLLLLGTQREAFHAMAACRRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDIPDHHRQLPQFTSPELEAPYSREEERQRLSDLYHRLHARLHSTSRPLRLIYHVAEKETLLAWVTSKFELYTCLSPLVTKAGAILVVTKLLRWVKKEEDRLFIRYPPKYSTPPATSTDQAAHNGLFTGL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 MON1 homolog B (yeast)
别名 HSRG1, KIAA0872, MON1 homolog B (yeast), MON1B, SAND2
计算分子量 547 aa, 59 kDa
观测分子量59 kDa
GenBank蛋白编号BC024277
基因名称 MON1B
Gene ID (NCBI) 22879
RRIDAB_2918734
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7L1V2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vacuolar fusion protein MON1 homolog B (MON1B) is also named as HSRG1, KIAA0872 and SAND2, and belongs to the MON1/SAND family. The membrane recruitment of MON1B, a homolog of yeast MON1 protein in mammals, is essential for vesicular fusion of early endosomes (PMID:26987402).

实验方案

Product Specific Protocols
IF protocol for MON1B antibody 67985-1-IgDownload protocol
WB protocol for MON1B antibody 67985-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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