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MON1B Monoclonal antibody

MON1B Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E7C6

Cat No : 67985-1-Ig

Print datasheet

Synonyms

HSRG1, KIAA0872, MON1 homolog B (yeast), MON1B, SAND2

验证数据展示

  • WB analysis using 67985-1-Ig

    WB analysis using 67985-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67985-1-Ig (MON1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of A549 using 67985-1-Ig

    WB analysis of A549 using 67985-1-Ig

    WB result of MON1B antibody (67985-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MON1B transfected A549 cells.

  • IF Staining of HeLa using 67985-1-Ig

    IF Staining of HeLa using 67985-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MON1B antibody (67985-1-Ig, Clone: 3E7C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, HepG2 cells, K-562 cells, SW480 cells, MCF-7 cells
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67985-1-Ig targets MON1B in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human
Immunogen MON1B fusion protein Ag9920 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name MON1 homolog B (yeast)
Synonyms HSRG1, KIAA0872, MON1 homolog B (yeast), MON1B, SAND2
Calculated Molecular Weight 547 aa, 59 kDa
Observed Molecular Weight59 kDa
GenBank Accession NumberBC024277
Gene Symbol MON1B
Gene ID (NCBI) 22879
RRIDAB_2918734
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7L1V2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vacuolar fusion protein MON1 homolog B (MON1B) is also named as HSRG1, KIAA0872 and SAND2, and belongs to the MON1/SAND family. The membrane recruitment of MON1B, a homolog of yeast MON1 protein in mammals, is essential for vesicular fusion of early endosomes (PMID:26987402).

实验方案

Product Specific Protocols
WB protocol for MON1B antibody 67985-1-IgDownload protocol
IF protocol for MON1B antibody 67985-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols