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验证数据展示

  • WB analysis using 30957-1-AP (same clone as 30957-1-PBS)

    WB analysis using 30957-1-AP (same clone as 30957-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30957-1-AP (MIF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30957-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 30957-1-AP (same clone as 30957-1-PBS)

    IF Staining of Neuro-2a using 30957-1-AP (same clone as 30957-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using MIF antibody (30957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30957-1-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 30957-1-AP (same clone as 30957-1-PBS)

    FC experiment of NIH/3T3 using 30957-1-AP (same clone as 30957-1-PBS)

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Mouse MIF (30957-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 30957-1-PBS in a different storage buffer formulation.

Mouse MIF Polyclonal antibody, PBS Only

MIF Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 30957-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

GIF

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30957-1-PBS targets MIF in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 macrophage migration inhibitory factor
别名 GIF
计算分子量13KD
观测分子量13 kDa
GenBank蛋白编号NM_010798.2
基因名称 Mif
Gene ID (NCBI) 17319
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP34884
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.

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