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验证数据展示

  • IHC staining of mouse liver using 30718-1-AP (same clone as 30718-1-PBS)

    IHC staining of mouse liver using 30718-1-AP (same clone as 30718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

  • WB analysis using 30718-1-AP (same clone as 30718-1-PBS)

    WB analysis using 30718-1-AP (same clone as 30718-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30718-1-AP (MGAT4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 30718-1-AP (same clone as 30718-1-PBS)

    IF Staining of U2OS using 30718-1-AP (same clone as 30718-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MGAT4B antibody (30718-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

MGAT4B Polyclonal antibody, PBS Only

MGAT4B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30718-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

GnT-IVb, GNT IVB, GNT IV, GlcNAc-T IVb, EC:2.4.1.145

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30718-1-PBS targets MGAT4B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag33769

Product name: Recombinant human MGAT4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 306-446 aa of BC009464

Sequence: KTYQHFTLEKAYLREDFFWAFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVDPAFGPLEALRLSIQTDSPVWVILSEIFLKKAD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
别名 GnT-IVb, GNT IVB, GNT IV, GlcNAc-T IVb, EC:2.4.1.145
计算分子量 548 aa, 63 kDa
观测分子量63 kDa
GenBank蛋白编号BC009464
基因名称 MGAT4B
Gene ID (NCBI) 11282
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQ53
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MGAT4B belongs to the N-Acetylglucosaminyltransferase-IV family and catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains (PMID: 17006639, 10372966). MGAT4B has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor to N-glycan biosynthesis (PMID:17006639).

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