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Host
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验证数据展示

  • IHC staining of mouse liver using 30718-1-AP (same clone as 30718-1-PBS)

    IHC staining of mouse liver using 30718-1-AP (same clone as 30718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

  • WB analysis using 30718-1-AP (same clone as 30718-1-PBS)

    WB analysis using 30718-1-AP (same clone as 30718-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30718-1-AP (MGAT4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 30718-1-AP (same clone as 30718-1-PBS)

    IF Staining of U2OS using 30718-1-AP (same clone as 30718-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MGAT4B antibody (30718-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

MGAT4B Polyclonal antibody, PBS Only

MGAT4B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30718-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

GnT-IVb, GNT IVB, GNT IV, GlcNAc-T IVb, EC:2.4.1.145

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30718-1-PBS targets MGAT4B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MGAT4B fusion protein Ag33769 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
别名 GnT-IVb, GNT IVB, GNT IV, GlcNAc-T IVb, EC:2.4.1.145
计算分子量 548 aa, 63 kDa
观测分子量63 kDa
GenBank蛋白编号BC009464
基因名称 MGAT4B
Gene ID (NCBI) 11282
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQ53
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MGAT4B belongs to the N-Acetylglucosaminyltransferase-IV family and catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains (PMID: 17006639, 10372966). MGAT4B has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor to N-glycan biosynthesis (PMID:17006639).