验证数据展示
经过测试的应用
| Positive WB detected in | 37°C incubated mouse liver tissue, human liver tissue |
| Positive IHC detected in | mouse liver tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30718-1-AP targets MGAT4B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag33769 Product name: Recombinant human MGAT4B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 306-446 aa of BC009464 Sequence: KTYQHFTLEKAYLREDFFWAFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVDPAFGPLEALRLSIQTDSPVWVILSEIFLKKAD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B |
| 别名 | GnT-IVb, GNT IVB, GNT IV, GlcNAc-T IVb, EC:2.4.1.145 |
| 计算分子量 | 548 aa, 63 kDa |
| 观测分子量 | 63 kDa |
| GenBank蛋白编号 | BC009464 |
| 基因名称 | MGAT4B |
| Gene ID (NCBI) | 11282 |
| RRID | AB_3086399 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UQ53 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MGAT4B belongs to the N-Acetylglucosaminyltransferase-IV family and catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains (PMID: 17006639, 10372966). MGAT4B has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor to N-glycan biosynthesis (PMID:17006639).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MGAT4B antibody 30718-1-AP | Download protocol |
| IHC protocol for MGAT4B antibody 30718-1-AP | Download protocol |
| WB protocol for MGAT4B antibody 30718-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
