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MGAT4B Polyclonal antibody

MGAT4B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 30718-1-AP

Print datasheet

Synonyms

GlcNAc T IVb, GNT IV, GNT IVB, MGAT4B

验证数据展示

  • IHC staining of mouse liver using 30718-1-AP

    IHC staining of mouse liver using 30718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 30718-1-AP

    WB analysis using 30718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30718-1-AP (MGAT4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected in37°C incubated mouse liver tissue, human liver tissue
Positive IHC detected inmouse liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30718-1-AP targets MGAT4B in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen MGAT4B fusion protein Ag33769 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
Synonyms GlcNAc T IVb, GNT IV, GNT IVB, MGAT4B
Calculated Molecular Weight 548 aa, 63 kDa
Observed Molecular Weight63 kDa
GenBank Accession NumberBC009464
Gene Symbol MGAT4B
Gene ID (NCBI) 11282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQ53
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MGAT4B belongs to the N-Acetylglucosaminyltransferase-IV family and catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains (PMID: 17006639, 10372966). MGAT4B has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor to N-glycan biosynthesis (PMID:17006639).

实验方案

Product Specific Protocols
WB protocol for MGAT4B antibody 30718-1-APDownload protocol
IHC protocol for MGAT4B antibody 30718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols