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验证数据展示

  • WB analysis using 29314-1-AP (same clone as 29314-1-PBS)

    WB analysis using 29314-1-AP (same clone as 29314-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29314-1-AP (METTL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29314-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 29314-1-AP (same clone as 29314-1-PBS)

    IHC staining of human lung cancer using 29314-1-AP (same clone as 29314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29314-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL3 antibody included

METTL3 Polyclonal antibody, PBS Only

METTL3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29314-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, Indirect ELISA

别名

EC:2.1.1.348, hMETTL3, Methyltransferase-like protein 3, MT A70, MTA70

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.

产品信息

29314-1-PBS targets METTL3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原

CatNo: Ag30638

Product name: Recombinant human METTL3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 285-485 aa of BC001650

Sequence: MKASDADRPCRKLHFRRIINKHTDESLGDCSFLNTCFHMDTCKYVHYEIDACMDSEAPGSKDHTPSQELALTQSVGGDSSADRLFPPQWICCDIRYLDVSILGKFAVVMADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 3
别名 EC:2.1.1.348, hMETTL3, Methyltransferase-like protein 3, MT A70, MTA70
计算分子量 64 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC001650
基因名称 METTL3
Gene ID (NCBI) 56339
RRIDAB_3086114
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86U44
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

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