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验证数据展示

  • WB analysis of HeLa using 23256-1-AP

    WB analysis of HeLa using 23256-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23256-1-AP (MEPE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 23256-1-AP

    IHC staining of human lung using 23256-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung slide using 23256-1-AP (MEPE Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 23256-1-AP

    IHC staining of human lung using 23256-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung slide using 23256-1-AP (MEPE Antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of HeLa using 23256-1-AP

    IP experiment of HeLa using 23256-1-AP

    IP result of anti-MEPE (IP:23256-1-AP, 4ug; Detection:23256-1-AP 1:1000) with HeLa cells lysate 4000ug.

  • IF Staining of HepG2 using 23256-1-AP

    IF Staining of HepG2 using 23256-1-AP

    Immunofluorescent analysis of HepG2 cells, using MEPE antibody 23256-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

MEPE Polyclonal antibody

MEPE Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Cat No. 23256-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IF, IHC, ELISA

别名

MEPE, OF45, Osteoblast/osteocyte factor 45

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23256-1-AP targets MEPE in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IF, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19758

Product name: Recombinant human MEPE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 176-525 aa of BC128158

Sequence: VLNIIPASMNYAKAHSKDKKKPQRDSQAQKSPVKSKSTHRIQHNIDYLKHLSKVKKIPSDFEGSGYTDLQERGDNDISPFSGDGQPFKDIPGKGEATGPDLEGKDIQTGFAGPSEAESTHLDTKKPGYNEIPEREENGGNTIGTRDETAKEADAVDVSLVEGSNDIMGSTNFKELPGREGNRVDAGSQNAHQGKVEFHYPPAPSKEKRKEGSSDAAESTNYNEIPKNGKGSTRKGVDHSNRNQATLNEKQRFPSKGKSQGLPIPSRGLDNEIKNEMDSFNGPSHENIITHGRKYHYVPHRQNNSTRNKGMPQGKGSWGRQPHSNRRFSSRRRDDSSESSDSGSSSESDGD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix extracellular phosphoglycoprotein
别名 MEPE, OF45, Osteoblast/osteocyte factor 45
计算分子量 525 aa, 58 kDa
观测分子量 98 kDa
GenBank蛋白编号BC128158
基因名称 MEPE
Gene ID (NCBI) 56955
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ76
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases. (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a full-length human MEPE protein detects a major band of 98 kDa.

实验方案

Product Specific Protocols
WB protocol for MEPE antibody 23256-1-APDownload protocol
IHC protocol for MEPE antibody 23256-1-APDownload protocol
IF protocol for MEPE antibody 23256-1-APDownload protocol
IP protocol for MEPE antibody 23256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols