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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of MCF-7 using 82893-1-RR (same clone as 82893-1-PBS)

    FC experiment of MCF-7 using 82893-1-RR (same clone as 82893-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human MELK (82893-1-RR, Clone:230106D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82893-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82893-1-RR

    Affinity and Kinetic Characterization of 82893-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82893-1-RR against Human MELK were performed. The affinity constant is below 1 pM.

    {{ptg:ProductFeaturesInfo}}

MELK Recombinant monoclonal antibody, PBS Only

MELK Uni-rAb® Recombinant Antibody for FC (Intra), Indirect ELISA
Cat No. 82893-1-PBS

产品说明书

CloneNo. 230106D4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), Indirect ELISA

别名

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MELK Ready-To-Use IHC Kit. MELK primary antibody included.

产品信息

82893-1-PBS targets MELK in FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1897

Product name: Recombinant human MELK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 302-651 aa of BC014039

Sequence: EDLISLWQYDHLTATYLLLLAKKARGKPVRLRLSSFSCGQASATPFTDIKSNNWSLEDVTASDKNYVAGLIDYDWCEDDLSTGAATPRTSQFTKYWTESNGVESKSLTPALCRTPANKLKNKENVYTPKSAVKNEEYFMFPEPKTPVNKNQHKREILTTPNRYTTPSKARNQCLKETPIKIPVNSTGTDKLMTGVISPERRCRSVELDLNQAHMEETPKRKGAKVFGSLERGLDKVITVLTRSKRKGSARDGPRRLKLHYNVTTTRLVNPDQLLNEIMSILPKKHVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQKPDVVGIRRQRLKGDAWVYKRLVEDILSSCKV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 maternal embryonic leucine zipper kinase
别名 KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2
计算分子量 651 aa, 75 kDa
GenBank蛋白编号BC014039
基因名称 MELK
Gene ID (NCBI) 9833
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14680
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.