验证数据展示
经过测试的应用
| Positive WB detected in | LPS and Brefeldin A treated RAW 264.7 cells |
| Positive FC (Intra) detected in | LPS and Brefeldin A treated RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
RMX00085 targets MCP-1/CCL2 in WB, FC (Intra), ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Multi-Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) ligand 2 |
| 别名 | Ccl2, MCP1, MCP-1, C-C motif chemokine 2, CCL 2 |
| 观测分子量 | 16-20 kDa |
| GenBank蛋白编号 | NM-011333 |
| 基因名称 | MCP-1/CCL2 |
| Gene ID (NCBI) | 20296 |
| RRID | AB_3750134 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | N/A |
| UNIPROT ID | P10148 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Monocyte chemotactic protein 1 (MCP-1), also known as CCL2, is chemotactic for monocyte/macrophage, B cell, and T cell, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for MCP-1/CCL2 antibody RMX00085 | Download protocol |
| WB protocol for MCP-1/CCL2 antibody RMX00085 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
