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MAP3K10 Polyclonal antibody

MAP3K10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 29985-1-AP

Print datasheet

Synonyms

MAP3K10, Mixed lineage kinase 2, MLK2, MST, Protein kinase MST

验证数据展示

  • WB analysis using 29985-1-AP

    WB analysis using 29985-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29985-1-AP (MAP3K10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29985-1-AP

    WB analysis using 29985-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29985-1-AP (MAP3K10 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29985-1-AP targets MAP3K10 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen MAP3K10 fusion protein Ag32419 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 10
Synonyms MAP3K10, Mixed lineage kinase 2, MLK2, MST, Protein kinase MST
Calculated Molecular Weight104KD
Observed Molecular Weight104-115 kDa
GenBank Accession NumberNM_002446
Gene Symbol MAP3K10
Gene ID (NCBI) 4294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02779
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K10, also named as MLK2 and MST, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K10 activates the JUN N-terminal pathway. MAP3K10 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. MAP3K10 can be detected 104-115 kDa by phosphorylation (PMID: 23178452).

实验方案

Product Specific Protocols
WB protocol for MAP3K10 antibody 29985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols