• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 32346-1-AP

    WB analysis using 32346-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32346-1-AP (Ly-6G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of mouse bone marrow cells using 32346-1-AP

    FC experiment of mouse bone marrow cells using 32346-1-AP

    1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Mouse Ly-6g Polyclonal Antibody (32346-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue), or 0.25 ug APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (APC-65140, Clone: RB6-8C5). Cells were not fixed.

Ly-6G Polyclonal antibody

Ly-6G Polyclonal Antibody for WB, FC, ELISA
Cat No. 32346-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, FC, ELISA

别名

Ly6g, Ly-6G.1, Ly 6G

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse bone marrow
Positive FC detected inmouse bone marrow cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32346-1-AP targets Ly-6G in WB, FC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, FC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphocyte antigen 6 complex, locus G
别名 Ly6g, Ly-6G.1, Ly 6G
计算分子量14 kDa
观测分子量 20-30 kDa
GenBank蛋白编号NM_001310438.1
基因名称 Ly-6G
Gene ID (NCBI) 546644
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP35461
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901).

实验方案

Product Specific Protocols
WB protocol for Ly-6G antibody 32346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols