验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse bladder tissue, rat bladder tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55300-1-AP targets LIMS2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | LIM and senescent cell antigen-like domains 2 |
| 别名 | LIM like protein 2, PINCH2, PINCH-2 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | NM_017980 |
| 基因名称 | LIMS2 |
| Gene ID (NCBI) | 55679 |
| RRID | AB_3670247 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z4I7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LIMS2, also known as PINCH2, is a member of a small family of focal adhesion proteins. LIMS2 contains five LIM domains, each domain forming two zinc fingers, which permit interactions that regulate cell shape and migration. Along with integrin-linked kinase and parvin, LIMS2 is a component of a complex that mediates multiple protein-protein interactions at adhesion sites between cells and the extracellular matrix (ECM) (PMID: 12167643). Mutations in LIMS2 are associated with a novel muscular dystrophy, severe cardiomyopathy and triangular tongues(PMID: 25589244).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LIMS2 antibody 55300-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |