验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, mouse kidney tissue, mouse liver tissue, mouse lung tissue, mouse spleen tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31796-1-AP targets LIAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag37082 Product name: Recombinant human C17orf97 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 100-200 aa of BC141806 Sequence: GDDKHLSQSLKSQPLSSSFHDILSPCKERGPKPEHRQSKVEKKHLPSDSSTVSLPDFAEIENLANRINESLRWDGILADPEAEKERIRIYKLNRRKRYRCL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 17 open reading frame 97 |
| 别名 | C17orf97, Ligand of ATE1 protein, Protein LIAT1 |
| 观测分子量 | 20-70 kDa |
| GenBank蛋白编号 | BC141806 |
| 基因名称 | C17orf97 |
| Gene ID (NCBI) | 400566 |
| RRID | AB_3670116 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6ZQX7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Liat1 (Ligand of Ate1) is a protein of unknown function that was originally discovered through its interaction with arginyl tRNA protein transferase 1 (Ate1), a component of the Arg/N-degron pathway of protein degradation.Liat1 participates in Liquid-liquid phase separation (LLPS), a process which enables the temporal and spatial regulation of important cellular processes such as RNA metbolism, genome organization, DNA repair, and transcription regulation. Liat1 interacts with itself, which means Liat1 can assemble into different species in nucleus or cytosol. Our WB is consistent with Arva A et al (PMID: 33443146).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for LIAT1 antibody 31796-1-AP | Download protocol |
| IHC protocol for LIAT1 antibody 31796-1-AP | Download protocol |
| WB protocol for LIAT1 antibody 31796-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
