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验证数据展示

  • WB analysis using 14600-1-AP (same clone as 14600-1-PBS)

    WB analysis using 14600-1-AP (same clone as 14600-1-PBS)

    Non-treated and Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14600-1-AP (same clone as 14600-1-PBS)

    IF Staining of HeLa using 14600-1-AP (same clone as 14600-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using LC3 antibody (14600-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 14600-1-AP (same clone as 14600-1-PBS)

    WB analysis of HeLa using 14600-1-AP (same clone as 14600-1-PBS)

    WB result of LC3 antibody (14600-1-AP; 1:6000; room temperature for 1.5 hours) with negative Control and LC3 knockout HeLa cells. This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

  • WB analysis using 14600-1-AP (same clone as 14600-1-PBS)

    WB analysis using 14600-1-AP (same clone as 14600-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 14600-1-AP (same clone as 14600-1-PBS)

    IHC staining of human liver using 14600-1-AP (same clone as 14600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LC3 antibody included
  • IHC staining of human liver using 14600-1-AP (same clone as 14600-1-PBS)

    IHC staining of human liver using 14600-1-AP (same clone as 14600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LC3 antibody included
  • FC experiment of HeLa using 14600-1-AP (same clone as 14600-1-PBS)

    FC experiment of HeLa using 14600-1-AP (same clone as 14600-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human LC3 (14600-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

LC3 Polyclonal antibody, PBS Only

LC3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 14600-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

MAP1LC3B, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, MAP1 light chain 3-like protein 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy LC3 Ready-To-Use IHC Kit. LC3 primary antibody included.

产品信息

14600-1-PBS targets LC3 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LC3 fusion protein Ag6144 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microtubule-associated protein 1 light chain 3 beta
别名 MAP1LC3B, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, MAP1 light chain 3-like protein 2
计算分子量 15 kDa
观测分子量 14-18 kDa
GenBank蛋白编号BC067797
基因名称 LC3B
Gene ID (NCBI) 81631
ENSEMBL Gene IDENSG00000140941
RRIDAB_2137737
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9GZQ8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.