• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human pancreas using 18294-1-AP

    IHC staining of human pancreas using 18294-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 18294-1-AP

    IHC staining of human pancreas using 18294-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18294-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 18294-1-AP

    IF Staining of HepG2 using 18294-1-AP

    Immunofluorescent analysis of HepG2 cells, using KRAS antibody 18294-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

KRAS-4B-specific Polyclonal antibody

KRAS-4B-specific Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 18294-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

KRAS-4B, KRAS, pan RAS, GTPase KRas, EC:3.6.5.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IHC detected inhuman pancreas tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18294-1-AP targets KRAS-4B-specific in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
别名 KRAS-4B, KRAS, pan RAS, GTPase KRas, EC:3.6.5.2
计算分子量 21 kDa
GenBank蛋白编号BC013572
基因名称 KRAS
Gene ID (NCBI) 3845
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01116
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. KRAS has two isoforms, 4A and 4B. The antibody is specific to KRAS, can't recognize NRAS and HRAS.

实验方案

Product Specific Protocols
IHC protocol for KRAS-4B-specific antibody 18294-1-APDownload protocol
IF protocol for KRAS-4B-specific antibody 18294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols