验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse kidney tissue, mouse testis tissue, rat heart tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32543-1-AP targets JAM3 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Eg2661 Product name: Recombinant Mouse JAM-3 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 30-241 aa of NM_023277.4 Sequence: EAVNLKSSNRNPVVHEFESVELSCIITDSQTSDPRIEWKKIQDGQTTYVYFDNKIQGDLAGRTDVFGKTSLRIWNVTRSDSAIYRCEVVALNDRKEVDEITIELIVQVKPVTPVCRIPAAVPVGKTATLQCQESEGYPRPHYSWYRNDVPLPTDSRANPRFQNSSFHVNSETGTLVFNAVHKDDSGQYYCIASNDAGAARCEGQDMEVYDLN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | junction adhesion molecule 3 |
| 别名 | JAM-2, JAM-3, JAM-C, Junctional adhesion molecule 3, Junctional adhesion molecule C |
| 计算分子量 | 35 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | NM_023277.4 |
| 基因名称 | Jam3 |
| Gene ID (NCBI) | 83964 |
| RRID | AB_3742650 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9D8B7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Junctional adhesion molecule 3 (JAM3) is a type I transmembrane glycoprotein containing two Ig-like domains, which is expressed in various tissues and plays a crucial role in cell junctions, cell polarity, and motility (PMID: 34292449; 28931565). It has been proposed that JAM3 participates in leukocyte-platelet interactions, as well as angiogenesis and brain development (PMID: 12208882; 23255084). JAM3 may also be a new marker for predicting the prognosis and immune functions of bladder cancer patients (PMID: 38400838).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for JAM3 antibody 32543-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
