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验证数据展示

  • IF Staining of human PBMCs using 68893-3-Ig

    IF Staining of human PBMCs using 68893-3-Ig

    Immunofluorescent analysis of un-fixed 5 µg/mL PHA ( 3 days) treated human PBMCs using Integrin alpha-E antibody (68893-3-Ig, Clone: 4B6G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005).

  • FC experiment of human PBMCs using 68893-3-Ig

    FC experiment of human PBMCs using 68893-3-Ig

    1x10^6 untreated human PBMCs (left) and 5 µg/mL PHA (3 days) treated human PBMCs (right) were surface stained with 0.2 μg Integrin Alpha-E Monoclonal Antibody (68893-3-Ig, Clone: 4B6G6) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Cells were not fixed.

CD103 Monoclonal antibody

CD103 Monoclonal Antibody for IF/ICC, FC, ELISA
Cat No. 68893-3-Ig

产品说明书

CloneNo. 4B6G6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC, ELISA

别名

4B6G6, HML 1 antigen, HML-1 antigen, HUMINAE, Integrin alpha E

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected in5 µg/mL PHA ( 3 days) treated human PBMCs
Positive FC detected in5 µg/mL PHA for 3 days human PBMCs

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68893-3-Ig targets CD103 in IF/ICC, FC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27410

Product name: Recombinant human Integrin alpha-E protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 147-456 aa of BC113436

Sequence: LLDPDARVDTGDCYSNKEGGGEDDVNTARQRRALEKEEEEDKEEEEDEEEEEAGTEIAIILDGSGSIDPPDFQRAKDFISNMMRNFYEKCFECNFALVQYGGVIQTEFDLRDSQDVMASLARVQNITQVGSVTKTASAMQHVLDSIFTSSHGSRRKASKVMVVLTDGGIFEDPLNLTTVINSPKMQGVERFAIGVGEEFKSARTARELNLIASDPDETHAFKVTNYMALDGLLSKLRYNIISMEGTVGDALHYQLAQIGFSAQILDERQVLLGAVGAFDWSGGALLYDTRSRRGRFLNQTAAAAADAEAA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
别名 4B6G6, HML 1 antigen, HML-1 antigen, HUMINAE, Integrin alpha E
计算分子量 1179 aa, 130 kDa
GenBank蛋白编号BC113436
基因名称 ITGAE
Gene ID (NCBI) 3682
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP38570
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrin alpha-E (CD103) is a member of the integrin family, specifically the αEβ7 heterodimer. It plays a crucial role in the immune system, particularly in the interaction between leukocytes and epithelial cells. Integrin alpha-E (CD103) is a critical component of the immune system, particularly in the context of mucosal immunity. Its ability to bind to E-cadherin and regulate the production of IFN-I in cDCs during viral infections highlights its multifaceted role in immune regulation and disease prevention. (PMID: 33584680)

实验方案

Product Specific Protocols
FC protocol for CD103 antibody 68893-3-IgDownload protocol
IF protocol for CD103 antibody 68893-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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