验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HuH-7 cells, Ramos cells, human peripheral blood platelets, mouse testis tissue |
Positive IHC detected in | human stomach tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30692-1-AP targets IQGAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | IQGAP2 fusion protein Ag33518 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | IQ motif containing GTPase activating protein 2 |
别名 | |
计算分子量 | 181 kDa |
观测分子量 | 181 kDa |
GenBank蛋白编号 | NM_006633 |
基因名称 | IQGAP2 |
Gene ID (NCBI) | 10788 |
RRID | AB_3086388 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q13576 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IQGAP2, a member of the IQGAP family, functions as a tumor suppressor in most of the cancers. IQGAP2 contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. IQGAP2 interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. It also acts as a tumor suppressor and has been found to play a role in regulating innate antiviral responses.
实验方案
Product Specific Protocols | |
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WB protocol for IQGAP2 antibody 30692-1-AP | Download protocol |
IHC protocol for IQGAP2 antibody 30692-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |