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验证数据展示

  • WB analysis using 31664-1-AP

    WB analysis using 31664-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31664-1-AP (INTS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 31664-1-AP

    WB analysis using 31664-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31664-1-AP (INTS6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 31664-1-AP

    IHC staining of human ovary cancer using 31664-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31664-1-AP (INTS6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

INTS6 Polyclonal antibody

INTS6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31664-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

HDB, DICE1, DDX26A, DDX26, DBI1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells, HuH-7 cells, L02 cells, PC-3 cells, LNCaP cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31664-1-AP targets INTS6 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 INTS6 fusion protein Ag36146 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 integrator complex subunit 6
别名 HDB, DICE1, DDX26A, DDX26, DBI1
计算分子量 100 kDa
观测分子量130 kDa
GenBank蛋白编号BC039829
基因名称 INTS6
Gene ID (NCBI) 26512
RRIDAB_3670067
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9UL03
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INTS6 is a component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing.

实验方案

Product Specific Protocols
WB protocol for INTS6 antibody 31664-1-APDownload protocol
IHC protocol for INTS6 antibody 31664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols