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验证数据展示

  • WB analysis using 32180-1-AP

    WB analysis using 32180-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32180-1-AP (IL23(IL12B+IL23A) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of Raji using 32180-1-AP

    IF Staining of Raji using 32180-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using IL23(IL12B+IL23A) antibody (32180-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IL23(IL12B+IL23A) Polyclonal antibody

IL23(IL12B+IL23A) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32180-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

IL 23, IL23 (IL12B+IL23A), IL-23, IL23

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, SW480 cells, U266 cells
Positive IF/ICC detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32180-1-AP targets IL23(IL12B+IL23A) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IL23(IL12B+IL23A)
别名 IL 23, IL23 (IL12B+IL23A), IL-23, IL23
计算分子量54 kDa, 37 kDa, 21 kDa
观测分子量54 kDa, 37 kDa
基因名称
Gene ID (NCBI)
RRIDAB_3670216
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP29460(IL12B)+linker+Q9NPF7(IL23A)
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-12 and IL-23 are heterodimeric cytokines with a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). The importance of IL-23 has been confirmed by clinical data, as monoclonal antibodies (MABs) targeting the p40 subunit (shared by IL-23 and IL-12), as well as p19 (specific for IL-23) were effective in therapy for psoriasis and psoriatic arthritis (PMID: 25754330; 18486740). This antibody can recognize the IL12B monomer (37 kDa) and the heterodimer (IL12B+IL23A, 54 kDa).

实验方案

Product Specific Protocols
WB protocol for IL23(IL12B+IL23A) antibody 32180-1-APDownload protocol
IF protocol for IL23(IL12B+IL23A) antibody 32180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols