Speedy™ Human IL-2 One-Step ELISA Kit
Sensitivity
0.8 pg/mL
Range
3.9-250 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IL-2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.8 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3558 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 91% | 80%-102% |
| Cell culture supernatant | 93% | 87%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 138.0 | 9.1 | 6.6 |
| 2 | 8 | 30.7 | 2.0 | 6.5 |
| 3 | 8 | 13.8 | 0.9 | 6.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 135.8 | 7.8 | 5.7 |
| 2 | 16 | 30.5 | 1.8 | 5.9 |
| 3 | 16 | 14.0 | 1.6 | 11.4 |
Background Information
Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of theIL-2Rβand IL-2Rγreceptor subunits.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL2, IL 2, interleukin 2, Interleukin-2, TCGF |
FAQ
1、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
