Speedy™ Human GPC1 One-Step ELISA Kit
Sensitivity
0.01 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
| Product name | Speedy™ Human GPC1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2817 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 110% | 104%-117% |
| Cell culture supernatant | 98% | 95%-103% |
| Urine | 101% | 98%-105% |
| Tissue lysate | 99% | 90%-109% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.48 | 0.07 | 1.28 |
| 2 | 8 | 1.32 | 0.09 | 6.82 |
| 3 | 8 | 0.61 | 0.02 | 3.28 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.71 | 0.45 | 7.88 |
| 2 | 16 | 1.30 | 0.07 | 5.38 |
| 3 | 16 | 0.64 | 0.04 | 6.25 |
Background Information
Glypicans are a family of HSPGs that are attached to the cell membrane by a glycosyl-phosphatidylinositol anchor. Glypicans are considered to have the ability to modulate the activities of HBGFs. The expression of glypicans is regulated temporally and spatially during development, suggesting they are involved in development and morphogenesis. To date, six glypicans (GPC1 to GPC6) have been identified in mammals. GPC1 is most ubiquitously expressed in adult tissues. GPC1 promotes efficient signaling by HBGFs and plays a critical role in cell growth and differentiation. GPC1 is involved in tumorigenesis and angiogenesis, and is frequently overexpressed in several types of tumors including pancreatic carcinoma, breast cancer and glioma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | glypican 1, FLJ38078, glypican, glypican1, Glypican-1 |
FAQ
1、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
