Speedy™ Human Factor IX/PTC One-Step ELISA Kit
Sensitivity
11.7 pg/mL
Range
109.5-7000 pg/mL
Reactivity
Human
Cat no : SE50116
Synonyms
F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Factor IX/PTC One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 11.7 pg/mL |
| Range | 109.5-7000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2158 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 106% | 84%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 3,564.2 | 104.1 | 2.9 |
| 2 | 8 | 819.6 | 14.8 | 1.8 |
| 3 | 8 | 425.1 | 7.7 | 1.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 3,663.4 | 147.2 | 4.0 |
| 2 | 16 | 838.7 | 26.4 | 3.1 |
| 3 | 16 | 441.8 | 19.8 | 4.5 |
Background Information
Factor IX, also known as PTC, circulates as an inactive zymogen until the proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Upon activation by Factor XIa or Factor VIIa/tissue factor complex, Factor IXa forms a complex with Factor VIIIa, calcium ions, and phospholipids on the surface of activated platelets. This complex, known as the tenase complex, efficiently activates Factor X to Factor Xa, leading to thrombin generation and fibrin clot formation. Deficiencies or mutations in Factor IX result in Hemophilia B, an X-linked recessive bleeding disorder.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain |
FAQ
1、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
