Speedy™ Human APEX1 One-Step ELISA Kit
Sensitivity
0.02 ng/mL
Range
0.625-40 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
| Product name | Speedy™ Human APEX1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 328 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 89% | 82%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 21.66 | 0.72 | 3.32 |
| 2 | 8 | 5.08 | 0.14 | 2.76 |
| 3 | 8 | 2.53 | 0.11 | 4.35 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 22.24 | 1.24 | 5.58 |
| 2 | 16 | 5.16 | 0.22 | 4.26 |
| 3 | 16 | 2.55 | 0.11 | 4.31 |
Background Information
APEX1 (Apurinic/Apyrimidinic Endonuclease 1), also known as APE1, HAP1, or Ref-1, is a multifunctional DNA repair enzyme. This protein is renowned for its bifunctional properties: on one hand, APEX1 is a core component of the base excision repair (BER) pathway, initiating DNA damage repair by cleaving apurinic/apyrimidinic (AP) sites to maintain genomic stability. On the other hand, APEX1 possesses redox activity, capable of reductively activating various transcription factors (such as AP-1, NF-κB, p53, HIF-1α, etc.) to regulate gene expression. Furthermore, this protein is involved in ribonucleotide reductase regulation, RNA processing, and mitochondrial DNA repair. APEX1 is highly expressed in various tumors and is closely associated with tumor drug resistance, radioresistance, and poor prognosis, making it a potential therapeutic target and prognostic marker. Its functional deficiency is linked to neurodegenerative diseases, cancer susceptibility, and aging.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | APE, APE1, APE-1, APEN, APEX |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
