验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, mouse liver tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33095-1-AP targets Hemopexin in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Eg2829 Product name: Recombinant Mouse Hpx/Hemopexin protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-460 aa of NM_017371.2 Sequence: SPLPTANGRVAEVENGTKPDSDVPEHCLDTWSFDAATMDHNGTMLFFKGEFVWRGHSGTRELISARWKNPITSVDAAFRGPDSVFLIKEDKVWVYPPEKKENGYPKLFQEEFPGIPYPPDAAVECHRGECQSEGVLFFQGNRKWFWDFATRTQKERSWSTVGNCTAALRWLERYYCFQGNKFLRFNPVTGEVPPRYPLDARDYFVSCPGRGHGRPRNGTAHGNSTHPMHSRCSPDPGLTALLSDHRGATYAFTGSHYWRLDSSRDGWHSWPIAHHWPQGPSTVDAAFSWDDKVYLIQGTQVYVFLTKGGNNLVSGYPKRLEKELGSPPGISLETIDAAFSCPGSSRLYVSSGRRLWWLDLKSGAQATWTEVSWPHEKVDGALCLDKSLGPNTCSSNGSSLYFIHGPNLYCYSSIDKLNAAKSLPQPQKVNSILGCSQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hemopexin |
| 别名 | Hpx, Hpxn |
| 计算分子量 | 51 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | NM_017371.2 |
| 基因名称 | Hpx |
| Gene ID (NCBI) | 15458 |
| RRID | AB_3742852 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q91X72 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Hemopexin antibody 33095-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
