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CoraLite® Plus 488-conjugated HLA-DPB1 Polyclonal antibody

HLA-DPB1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-11298

Print datasheet

Synonyms

DPB1, HLA DP1B, HLA DPB1, HLA-DPB1, MHC class II antigen DPB1

验证数据展示

  • FC experiment of Daudi using CL488-11298

    FC experiment of Daudi using CL488-11298

    1X10^6 Daudi cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human HLA-DPB1 (CL488-11298) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inDaudi cells
For other applications, we recommend the unconjugated version of this antibody, 11298-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11298 targets HLA-DPB1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra)
Tested Reactivity human
Immunogen HLA-DPB1 fusion protein Ag1815 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name major histocompatibility complex, class II, DP beta 1
Synonyms DPB1, HLA DP1B, HLA DPB1, HLA-DPB1, MHC class II antigen DPB1
Calculated Molecular Weight 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC015000
Gene Symbol HLA-DPB1
Gene ID (NCBI) 3115
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04440
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 HLA-DPB1 antibody CL488-11298Download protocol
Standard Protocols
Click here to view our Standard Protocols