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验证数据展示

  • WB analysis using 68157-1-Ig (same clone as 68157-1-PBS)

    WB analysis using 68157-1-Ig (same clone as 68157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68157-1-Ig (HIBCH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

    WB analysis of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

    WB result of HIBCH antibody (68157-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIBCH transfected HepG2 cells. This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

    IF Staining of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (68157-1-Ig, Clone: 1B7B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

HIBCH Monoclonal antibody, PBS Only

HIBCH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68157-1-PBS

产品说明书

CloneNo. 1B7B8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, Indirect ELISA

别名

HIBYL COA H, HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HIBCH Ready-To-Use IHC Kit. HIBCH primary antibody included.

产品信息

68157-1-PBS targets HIBCH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 HIBCH fusion protein Ag6473 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 3-hydroxyisobutyryl-Coenzyme A hydrolase
别名 HIBYL COA H, HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase
计算分子量 43 kDa
观测分子量38 kDa
GenBank蛋白编号BC067822
基因名称 HIBCH
Gene ID (NCBI) 26275
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6NVY1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).