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验证数据展示

  • WB analysis using 86747-1-RR (same clone as 86747-1-PBS)

    WB analysis using 86747-1-RR (same clone as 86747-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86747-1-RR (GUF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86747-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 86747-1-RR (same clone as 86747-1-PBS)

    IP experiment of HepG2 using 86747-1-RR (same clone as 86747-1-PBS)

    IP result of anti-GUF1 (IP:86747-1-RR, 4ug; Detection:86747-1-RR 1:2000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 86747-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86747-1-RR

    Affinity and Kinetic Characterization of 86747-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86747-1-RR against Human GUF1 were performed. The affinity constant is 9.10 pM.

GUF1 Recombinant monoclonal antibody, PBS Only

GUF1 Uni-rAb® Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 86747-1-PBS

产品说明书

CloneNo. 251449D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, Indirect ELISA

别名

EC:3.6.5.-, EF-4, Elongation factor 4 homolog, GTPase GUF1, Ribosomal back-translocase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86747-1-PBS targets GUF1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11579

Product name: Recombinant human GUF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 318-669 aa of BC036768

Sequence: VGYLIAGMKDVTEAQIGDTLCLHKQPVEPLPGFKSAKPMVFAGMYPLDQSEYNNLKSAIEKLTLNDSSVTVHRDSSLALGAGWRLGFLGLLHMEVFNQRLEQEYNASVILTTPTVPYKAVLSSSKLIKEHREKEITIINPAQFPDKSKVTEYLEPVVLGTIITPDEYTGKIMMLCEARRAVQKNMIFIDQNRVMLKYLFPLNEIVVDFYDSLKSLSSGYASFDYEDAGYQTAELVKMDILLNGNTVEELVTVVHKDKAHSIGKAICERLKDSLPRQLFEIAIQAAIGSKIIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKKLRKIGNVEVPKDAFIKVLKTQSSK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GUF1 GTPase homolog (S. cerevisiae)
别名 EC:3.6.5.-, EF-4, Elongation factor 4 homolog, GTPase GUF1, Ribosomal back-translocase
计算分子量 669 aa, 74 kDa
观测分子量68-70 kDa
GenBank蛋白编号BC036768
基因名称 GUF1
Gene ID (NCBI) 60558
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8N442
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes.

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