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  • KD/KO Validated

GSDMD Polyclonal antibody

GSDMD Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 20770-1-AP

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Synonyms

Gasdermin-D, C-terminal, Gasdermin-D, gasderminD, Gasdermin domain-containing protein 1, gasdermin D



经过测试的应用

Positive WB detected inA431 cells, NIH/3T3 cells, Jurkat cells, C6 cells, rat liver tissue, rat brain tissue
Positive IP detected inTHP-1 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20770-1-AP targets GSDMD in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, bovine, hamster
Immunogen GSDMD fusion protein Ag14757 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gasdermin D
Synonyms Gasdermin-D, C-terminal, Gasdermin-D, gasderminD, Gasdermin domain-containing protein 1, gasdermin D
Calculated Molecular Weight 484 aa, 53 kDa
Observed Molecular Weight 50-53 kDa
GenBank Accession NumberBC008904
Gene Symbol GSDMD
Gene ID (NCBI) 79792
RRIDAB_10696319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57764
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

实验方案

Product Specific Protocols
WB protocol for GSDMD antibody 20770-1-APDownload protocol
IF protocol for GSDMD antibody 20770-1-APDownload protocol
IP protocol for GSDMD antibody 20770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.

Authors - Xing Liu
  • KD Validated
humanWB

ACS Nano

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Authors - Zhiwei Liao
humanWB

Acta Pharm Sin B

Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.

Authors - Xiaowei Zhang
humanWB

Acta Pharm Sin B

A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer

Authors - Yang Liu
HumanWB

Cell Res

Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion.

Authors - Wan-Ting He
humanWB

J Exp Med

Protein kinase D at the Golgi controls NLRP3 inflammasome activation.

Authors - Zhirong Zhang
  • KO Validated