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验证数据展示

  • WB analysis using 30047-1-AP

    WB analysis using 30047-1-AP

    HeLa cells lysates were subjected to SDS PAGE followed by western blot with 30047-1-AP (GPSN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 30047-1-AP

    IF Staining of U-251 using 30047-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using GPSN2 antibody (30047-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

GPSN2 Polyclonal antibody

GPSN2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30047-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Trans-2,3-enoyl-CoA reductase, TER, TECR, Synaptic glycoprotein SC2, SC2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30047-1-AP targets GPSN2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 GPSN2 fusion protein Ag32598 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycoprotein, synaptic 2
别名 Trans-2,3-enoyl-CoA reductase, TER, TECR, Synaptic glycoprotein SC2, SC2
计算分子量 36 kDa
观测分子量32-36 kDa
GenBank蛋白编号BC002425
基因名称 GPSN2
Gene ID (NCBI) 9524
RRIDAB_3086219
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZ01
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycoprotein synaptic 2 (GPSN2), also known as TER and TECR, functions as a trans-2,3-enoyl-CoA reductase and reduces long and very long chain fatty acid trans-2,3-enoyl-CoA substrates (PMID: 12482854).

实验方案

Product Specific Protocols
WB protocol for GPSN2 antibody 30047-1-APDownload protocol
IF protocol for GPSN2 antibody 30047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols