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验证数据展示

  • WB analysis using 12659-1-AP (same clone as 12659-1-PBS)

    WB analysis using 12659-1-AP (same clone as 12659-1-PBS)

    mouse brain lysates were subjected to SDS PAGE followed by western blot with 12659-1-AP (GPR155 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12659-1-AP (same clone as 12659-1-PBS)

    IP experiment of mouse brain using 12659-1-AP (same clone as 12659-1-PBS)

    IP result of anti-GPR155 (IP:12659-1-AP, 4ug; Detection:12659-1-AP 1:300) with mouse brain tissue lysate 3000ug. This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 12659-1-AP (same clone as 12659-1-PBS)

    IHC staining of human brain using 12659-1-AP (same clone as 12659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 12659-1-AP (same clone as 12659-1-PBS)

    IHC staining of human brain using 12659-1-AP (same clone as 12659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12659-1-AP (GPR155 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12659-1-PBS in a different storage buffer formulation.

GPR155 Polyclonal antibody, PBS Only

GPR155 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12659-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12659-1-PBS targets GPR155 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3328

Product name: Recombinant human GPR155 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 540-870 aa of BC028730

Sequence: MCMNQTAQAGGYEGFDQSQSHKVVEPGNTAFEESPAPVNEPELFTSSIPETSCCSCSMGNGELHCPSIEPIANTSTSEPVIPSFEKNNHCVSRCNSQSCILAQEEEQYLQSGDQQLTRHVLLCLLLIIGLFANLYSCLWWLFNQEPGRLYVELQFFCAVFNFGQGFISFGIFGLDKHLIILPFKRRLEFLWNNKDTAENRDSPVSEEIKMTCQQFIHYHRDLCIRNIVKERRCGAKTSAGTFCGCDLVSWLIEVGLASDRGEAVIYGDRLVQGGVIQHITNEYEFRDEYLFYRFLQKSPEQSPPAINANTLQQERYKEIEHSSPPSHSPKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 155
别名
计算分子量 870 aa, 97 kDa
观测分子量 97 kDa, 75 kDa
GenBank蛋白编号BC028730
基因名称 GPR155
Gene ID (NCBI) 151556
RRIDAB_2877869
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z3F1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The G Protein-coupled receptor 155 (GPR155), also known as Lysosomal cholesterol signaling protein (LYCHOS), is a cholesterol receptor on the lysosome. GRR155 plays a role in the cholesterol-sensing lysosomal pathway and couples cholesterol concentration to MTORC1-dependent anabolic signaling (PMID: 36007018). GPR155 may represent a biomarker for diagnosing and predicting hematogenous metastasis of gastric cancer (PMID: 28165032).

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