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GPBAR1 Polyclonal antibody

GPBAR1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26739-1-AP

Print datasheet

Synonyms

BG37, GPBAR1, GPCR, GPCR19, GPR131, hBG37, hGPCR19, M BAR, TGR5

验证数据展示

  • WB analysis using 26739-1-AP

    WB analysis using 26739-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26739-1-AP (GPBAR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 26739-1-AP

    IHC staining of mouse brain using 26739-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26739-1-AP (GPBAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCaco-2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26739-1-AP targets GPBAR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen GPBAR1 fusion protein Ag24893 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled bile acid receptor 1
Synonyms BG37, GPBAR1, GPCR, GPCR19, GPR131, hBG37, hGPCR19, M BAR, TGR5
Calculated Molecular Weight 35 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC033625
Gene Symbol GPBAR1
Gene ID (NCBI) 151306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDU6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPBAR1 (G-protein coupled bile acid receptor 1), also known as TGR5 or M-BAR, is a cell-surface receptor for bile acid which belongs to the G-protein coupled receptor 1 family (20236244). GPBAR1 gene expression is widely distributed, including endocrine glands, adipocytes, muscles, immune organs, spinal cord, and the enteric nervous system (24411485, 20665558, 22521118). GPBAR1 activation has some important biological effects such as anti-inflammatory, energy homeostasis and metabolism, anti-apoptotic, and choleretic functions (24411485, 22521118). In addition, GPBAR1 is related to several diseases, such as Metabolic and cardiovascular disorders, Hepatic and pancreatic disorders, Inflammatory bowel diseases, Gastrointestinal cancer, and so on (24411485).

实验方案

Product Specific Protocols
WB protocol for GPBAR1 antibody 26739-1-APDownload protocol
IHC protocol for GPBAR1 antibody 26739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols