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Host
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Species Reactivity
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Conjugate
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Compatible Species
0
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验证数据展示

  • WB analysis using 13493-1-AP (same clone as 13493-1-PBS)

    WB analysis using 13493-1-AP (same clone as 13493-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13493-1-AP (GP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13493-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 13493-1-AP (same clone as 13493-1-PBS)

    IHC staining of mouse pancreas using 13493-1-AP (same clone as 13493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13493-1-AP (GP2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13493-1-PBS in a different storage buffer formulation.

GP2 Polyclonal antibody, PBS Only

GP2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13493-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Pancreatic zymogen granule membrane protein GP-2, ZAP75

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

13493-1-PBS targets GP2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4317

Product name: Recombinant human GP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-360 aa of BC032693

Sequence: MERMVGSGLLWLALVSCILTQASAVQRDPSTVEDKCEKACRPEEECLALNSTWGCFCRQDLNSSDVHSLQPQLDCGPREIKVKVDKCLLGGLGLGEEVIAYLRDPNCSSILQTEERNWVSVTSPVQASACRNILERNQTHAIYKNTLSLVNDFIIRDTILNINFQCAYPLDMKVSLQAALQPIVSSLNVSVDGNGEFIVRMALFQDQNYTNPYQGDAVELSVESVLYVGAILEQGDTSRFNLVLRNCYATPTEDKADLVKYFIIRNSCSNQRDSTIHVEENGQSSESRFSVQMFMFAGHYDLVFLHCEIHLCDSLNEQCQPSCSRSQVRSEVPAIDLARVLDLGPITRRGAQSPGVMNGT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycoprotein 2 (zymogen granule membrane)
别名 Pancreatic zymogen granule membrane protein GP-2, ZAP75
计算分子量 530 aa, 58 kDa
观测分子量75-85 kDa
GenBank蛋白编号BC032693
基因名称 GP2
Gene ID (NCBI) 2813
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55259
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pancreatic secretory granule membrane major glycoprotein (GP2) is the most abundant membrane component of zymogen granules secreted by pancreatic acinar cells (PMID: 8190715). GP2 associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. It is released from the membrane of mature zymogen granules by an enzymatic mechanism (PMID: 2011597). GP2 plays a role in defense against adhesive and invasive commensal bacteria during intestinal inflammation (PMID: 33594081). GP2 is preferentially expressed in acinar cell carcinomas of the pancreas but the glycoprotein can rarely also be expressed in a variety of other tumor entities (PMID: 36137400). The apparent molecular weight of GP2 is 70-100 kDa, which is larger than the calculated molecular weight, due to glycosylation (PMID: 19627615; 30186250).