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验证数据展示

  • WB analysis using 13493-1-AP

    WB analysis using 13493-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13493-1-AP (GP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

GP2 Polyclonal antibody

GP2 Polyclonal Antibody for WB, ELISA
Cat No. 13493-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

ZAP75, Pancreatic zymogen granule membrane protein GP-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat pancreas tissue, mouse pancreas tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13493-1-AP targets GP2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GP2 fusion protein Ag4317 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycoprotein 2 (zymogen granule membrane)
别名 ZAP75, Pancreatic zymogen granule membrane protein GP-2
计算分子量 530 aa, 58 kDa
观测分子量75-85 kDa
GenBank蛋白编号BC032693
基因名称 GP2
Gene ID (NCBI) 2813
RRIDAB_3669164
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55259
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pancreatic secretory granule membrane major glycoprotein (GP2) is the most abundant membrane component of zymogen granules secreted by pancreatic acinar cells (PMID: 8190715). GP2 associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. It is released from the membrane of mature zymogen granules by an enzymatic mechanism (PMID: 2011597). GP2 plays a role in defense against adhesive and invasive commensal bacteria during intestinal inflammation (PMID: 33594081). GP2 is preferentially expressed in acinar cell carcinomas of the pancreas but the glycoprotein can rarely also be expressed in a variety of other tumor entities (PMID: 36137400). The apparent molecular weight of GP2 is 70-100 kDa, which is larger than the calculated molecular weight, due to glycosylation (PMID: 19627615; 30186250).

实验方案

Product Specific Protocols
WB protocol for GP2 antibody 13493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols