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验证数据展示

  • WB analysis of rat brain using 13625-1-AP (same clone as 13625-1-PBS)

    WB analysis of rat brain using 13625-1-AP (same clone as 13625-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 13625-1-AP (same clone as 13625-1-PBS)

    IHC staining of human lung cancer using 13625-1-AP (same clone as 13625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13625-1-AP (GMFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 13625-1-AP (same clone as 13625-1-PBS)

    IHC staining of human lung cancer using 13625-1-AP (same clone as 13625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13625-1-AP (GMFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse spleen using 13625-1-AP (same clone as 13625-1-PBS)

    IP experiment of mouse spleen using 13625-1-AP (same clone as 13625-1-PBS)

    IP result of anti-GMFG (IP:13625-1-AP, 4ug; Detection:13625-1-AP 1:600) with mouse spleen tissue lysate 2000 ug. This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GMFG Polyclonal antibody, PBS Only

GMFG Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13625-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

Glia maturation factor gamma, glia maturation factor, gamma, GMF GAMMA, GMF-gamma

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13625-1-PBS targets GMFG in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4538

Product name: Recombinant human GMFG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-141 aa of BC032819

Sequence: SDSLVVCEVDPELTEKLRKFRFRKETDNAAIIMKVDKDRQMVVLEEEFQNISPEELKMELPERQPRFVVYSYKYVHDDGRVSYPLCFIFSSPVGCKPEQQMMYAGSKNRLVQTAELTKVFEIRTTDDLTEAWLQEKLSFFR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glia maturation factor, gamma
别名 Glia maturation factor gamma, glia maturation factor, gamma, GMF GAMMA, GMF-gamma
计算分子量 141 aa, 17 kDa
观测分子量 17 kDa
GenBank蛋白编号BC032819
基因名称 GMFG
Gene ID (NCBI) 9535
RRIDAB_2110929
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60234
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GMFG, is a highly conserved brain-specific protein that belongs to the GMF subfamily of the larger Actin-binding protein ADF family. GMFG is preferentially expressed in blood vessel cells and immune cells and its ectopic expression enhances cellular functions such as tube-formation and migration in vitro. GMFG mediates neutrophil and T-lymphocyte migration via regulation of actin cytoskeletal reorganization. GMFG also acts as an intracellular regulator of stress-activated signal transduction by demonstrating activation of p38 MAP kinase and transcription factor NF-kB in astrocytes.

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