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验证数据展示

  • WB analysis of HeLa using 10356-1-AP (same clone as 10356-1-PBS)

    WB analysis of HeLa using 10356-1-AP (same clone as 10356-1-PBS)

    WB result of GGA2 antibody (10356-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GGA2 transfected HeLa cells. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 10356-1-AP (same clone as 10356-1-PBS)

    WB analysis of mouse brain using 10356-1-AP (same clone as 10356-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10356-1-AP (same clone as 10356-1-PBS)

    IHC staining of human colon cancer using 10356-1-AP (same clone as 10356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10356-1-AP (GGA2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 10356-1-AP (same clone as 10356-1-PBS)

    IHC staining of human stomach cancer using 10356-1-AP (same clone as 10356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10356-1-AP (GGA2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 10356-1-AP (same clone as 10356-1-PBS)

    IF Staining of HeLa using 10356-1-AP (same clone as 10356-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GGA2 antibody (10356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GGA2 Polyclonal antibody, PBS Only

GGA2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10356-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

10356-1-PBS targets GGA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GGA2 fusion protein Ag0370 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi associated, gamma adaptin ear containing, ARF binding protein 2
别名
计算分子量 67 kDa
观测分子量 67 kDa
GenBank蛋白编号BC000284
基因名称 GGA2
Gene ID (NCBI) 23062
RRIDAB_2110735
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJY4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GGA2, also known as VEAR, belongs to the GGA protein family and regulates the trafficking of proteins between the trans-Golgi network and the lysosome. GGA2 has an amino-terminal VHS domain in its N terminus which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.