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验证数据展示

  • WB analysis of MCF-7 using 22343-1-AP (same clone as 22343-1-PBS)

    WB analysis of MCF-7 using 22343-1-AP (same clone as 22343-1-PBS)

    WB result of GATA3 antibody (22343-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA3 transfected MCF-7 cells. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse thymus using 22343-1-AP (same clone as 22343-1-PBS)

    WB analysis of mouse thymus using 22343-1-AP (same clone as 22343-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

  • WB analysis using 22343-1-AP (same clone as 22343-1-PBS)

    WB analysis using 22343-1-AP (same clone as 22343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22343-1-AP (GATA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 22343-1-AP (same clone as 22343-1-PBS)

    IF Staining of MCF-7 using 22343-1-AP (same clone as 22343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (22343-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 22343-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GATA3 Polyclonal antibody, PBS Only

GATA3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 22343-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

GATA binding factor 3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

22343-1-PBS targets GATA3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag17833

Product name: Recombinant human GATA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-262 aa of BC006793

Sequence: MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSHSRGSMTALGGASSSTHHPITTYPPYVPEYSSGLFPPSSLLGGSPTGFGCKSRPKARSSTEGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GATA binding protein 3
别名 GATA binding factor 3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3
计算分子量 443 aa, 48 kDa
观测分子量 37 kDa and 48 kDa
GenBank蛋白编号BC006793
基因名称 GATA3
Gene ID (NCBI) 2625
RRIDAB_2879082
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23771
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Trans-acting T-cell-specific transcription factor, also known as GATA-3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin and mammary gland (PMID: 25148023). GATA-3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full length GATA3 protein contains 444 aa corresponding to the molecular weights of 48,0 kDa, and the truncated protein was deteced at approximately 37 kDa (PMID: 24758297).