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CoraLite®650-conjugated GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for
Cat No. CL650-60004

产品说明书

CloneNo. 1E6D9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, yeast, plant, zebrafish

应用

and More (1)

G3PD, GAPD, GAPDH, OK/SW cl.12

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60004-1-Ig

发表文章中的应用

WBSee 1 publications below

产品信息

CL650-60004 targets GAPDH in WB applications and shows reactivity with human, mouse, rat, yeast, plant, zebrafish samples.

经测试应用 Application Description
文献引用应用WB
经测试反应性 human, mouse, rat, yeast, plant, zebrafish
文献引用反应性human, mouse
免疫原 GAPDH fusion protein Ag0766 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glyceraldehyde-3-phosphate dehydrogenase
别名 G3PD, GAPD, GAPDH, OK/SW cl.12
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004109
基因名称 GAPDH
Gene ID (NCBI) 2597
RRIDAB_2920461
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04406
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the molecular weight of GAPDH?

The molecular weight of GAPDH is 36 kD.


What is GAPDH?

GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" 

protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic 

production of ATP and pyruvate (PMID: 21895736).


What is the function of GAPDH?

GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even 

critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH 

is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting 

as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in 

transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 

11035021).


Where in the cell is GAPDH localized?

GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus 

(PMIDs: 21895736, 12829261).


Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear 

functions?

Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is 

useful as a control for Western blots and RT-PCR.


What are the known protein-protein interactions of GAPDH?

GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of 

these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).


Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.


实验方案

Product Specific Protocols
FC protocol for CL Plus 647 GAPDH antibody CL650-60004Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Biol Direct

An integrated investigation of mitochondrial genes in COPD reveals the causal effect of NDUFS2 by regulating pulmonary macrophages

Authors - Xiaoli Zou

Cell Rep Med

Interferon-γ-stimulated antigen-presenting cancer-associated fibroblasts hinder neoadjuvant chemoimmunotherapy efficacy in lung cancer

Authors - Zhengqi Cao
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