CoraLite® Plus 647-conjugated GAPDH Monoclonal antibody
GAPDH Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, yeast, plant, zebrafish
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1E6D9
验证数据展示
经过测试的应用
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Sample-dependent, check data in validation data gallery | |
产品信息
CL650-60004 targets GAPDH in FC (Intra) applications and shows reactivity with human, mouse, rat, yeast, plant, zebrafish samples.
| Tested Applications | FC (Intra) |
| Tested Reactivity | human, mouse, rat, yeast, plant, zebrafish |
| Immunogen | GAPDH fusion protein Ag0766 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms | G3PD, GAPD, GAPDH, OK/SW cl.12 |
| Calculated molecular weight | 36 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC004109 |
| Gene symbol | GAPDH |
| Uniprot | P04406 |
| Gene ID (NCBI) | 2597 |
| RRID | AB_2920461 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
What is the molecular weight of GAPDH?
The molecular weight of GAPDH is 36 kD.
What is GAPDH?
GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping"
protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic
production of ATP and pyruvate (PMID: 21895736).
What is the function of GAPDH?
GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even
critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH
is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting
as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in
transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407,
11035021).
Where in the cell is GAPDH localized?
GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus
(PMIDs: 21895736, 12829261).
Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear
functions?
Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is
useful as a control for Western blots and RT-PCR.
What are the known protein-protein interactions of GAPDH?
GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of
these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).
Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 GAPDH antibody CL650-60004 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
