验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
31553-1-AP targets FYB in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | FYB fusion protein Ag35929 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | FYN binding protein (FYB-120/130) |
别名 | SLAP130, SLAP 130, p120/p130, FYB 120/130, ADAP |
计算分子量 | 85kDa,783aa |
观测分子量 | 110 kDa |
GenBank蛋白编号 | NM_199335.3 |
基因名称 | FYB |
Gene ID (NCBI) | 2533 |
RRID | AB_3670034 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity Purification |
UNIPROT ID | O15117 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FYN binding protein (FYB-120/130), also known as FYB, ADAP (Adhesion and degranulation-promoting adapter protein), and SLAP-130 (SLP-76-associated phosphoprotein) is a protein that is encoded by the FYB gene in humans. The protein is highly expressed in T cells, NK cells, bone marrow cells, and platelets, responsible for the signal transduction upon T cell receptor (TCR) stimulation with integrin activation.
实验方案
Product Specific Protocols | |
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WB protocol for FYB antibody 31553-1-AP | Download protocol |
IHC protocol for FYB antibody 31553-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |