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验证数据展示

  • WB analysis using 31553-1-AP

    WB analysis using 31553-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 31553-1-AP (FYB antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis using 31553-1-AP

    WB analysis using 31553-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31553-1-AP (FYB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 31553-1-AP

    IHC staining of human ovary cancer using 31553-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31553-1-AP (FYB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FYB Polyclonal antibody

FYB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31553-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ADAP, FYB 120/130, p120/p130, SLAP 130, SLAP130

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31553-1-AP targets FYB in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35929

Product name: Recombinant human FYB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 600-783 aa of NM_199335.3

Sequence: EQDDISSHSQSGSGGIFPPPPDDDIYDGIEEEDADDGFPAPPKQLDMGDEVYDDVDTSDFPVSSAEMSQGTNVGKAKTEEKDLKKLKKQEKEEKDFRKKFKYDGEIRVLYSTKVTTSITSKKWGTRDLQVKPGESLEVIQTTDDTKVLCRNEEGKYGYVLRSYLADNDGEIYDDIADGCIYDND

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FYN binding protein (FYB-120/130)
别名 ADAP, FYB 120/130, p120/p130, SLAP 130, SLAP130
计算分子量85kDa，783aa
观测分子量110 kDa
GenBank蛋白编号NM_199335.3
基因名称 FYB
Gene ID (NCBI) 2533
RRIDAB_3670034
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO15117
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FYN binding protein (FYB-120/130), also known as FYB, ADAP (Adhesion and degranulation-promoting adapter protein), and SLAP-130 (SLP-76-associated phosphoprotein) is a protein that is encoded by the FYB gene in humans. The protein is highly expressed in T cells, NK cells, bone marrow cells, and platelets, responsible for the signal transduction upon T cell receptor (TCR) stimulation with integrin activation.

实验方案

Product Specific Protocols
IHC protocol for FYB antibody 31553-1-APDownload protocol
WB protocol for FYB antibody 31553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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