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验证数据展示

  • WB analysis using 68058-1-Ig

    WB analysis using 68058-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68058-1-Ig (FXYD6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of mouse cerebellum using 68058-1-Ig

    IHC staining of mouse cerebellum using 68058-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 68058-1-Ig

    IHC staining of mouse cerebellum using 68058-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68058-1-Ig (FXYD6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of COLO 320 using 68058-1-Ig

    WB analysis of COLO 320 using 68058-1-Ig

    WB result of FXYD6 antibody (68058-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FXYD6 transfected COLO 320 cells.

  • IF Staining of PC-12 using 68058-1-Ig

    IF Staining of PC-12 using 68058-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using FXYD6 antibody (68058-1-Ig, Clone: 2H1B7 ) at dilution of 1:1000 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3).

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FXYD6 Monoclonal antibody

FXYD6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68058-1-Ig

产品说明书

CloneNo. 2H1B7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit, chicken

应用

WB, IHC, IF/ICC, ELISA

别名

2H1B7, FXYD domain-containing ion transport regulator 6, Phosphohippolin, UNQ521/PRO1056

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-12 cells, COLO 320 cells, rat cerebellum tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, mouse cerebellum tissue
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68058-1-Ig targets FXYD6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit, chicken
免疫原

CatNo: Ag8538

Product name: Recombinant human FXYD6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-95 aa of BC018652

Sequence: KEKEMDPFHYDYQTLRIGGLVFAVVLFSVGILLILSRRCKCSFNQKPRAPGDEEAQVENLITANATEPQKAEN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 FXYD domain containing ion transport regulator 6
别名 2H1B7, FXYD domain-containing ion transport regulator 6, Phosphohippolin, UNQ521/PRO1056
计算分子量 95 aa, 11 kDa
观测分子量20 kDa
GenBank蛋白编号BC018652
基因名称 FXYD6
Gene ID (NCBI) 53826
RRIDAB_2918799
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9H0Q3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The FXYD family is a group of small single-span transmembrane proteins characterized by a signature sequence containing an FXYD motif, two conserved glycines and a serine residue. Members of the FXYD family, including FXYD1 (phospholemman), FXYD2 (gamma subunit of Na,K-ATPase), FXYD3 (Mat8), FXYD4 (CHIF), FXYD5 (RIC), FXYD6 (phosphohippolin) and FXYD7, are tissue specific regulators of the Na,K-ATPase. FXYD6 is primarily expressed in the brain. It modulates the kinetic activity of Na,K-ATPase and has long-term physiological importance in maintaining cation homeostasis. It may play a role in endolymph composition and has a potential important role in neuronal excitability of the CNS during postnatal development and in the adult brain. On the SDS-PAGE FXYD6 migrates with an apparent molecular weight of approximately 20 kDa, which is larger than the calculated molecular weight of 10.5 kDa (PMID: 15193427; 17209044). The gene encodes FXYD6 is located on chromosome 11q23.3, and it might be a susceptibility gene of schizophrenia.

实验方案

Product Specific Protocols
IF protocol for FXYD6 antibody 68058-1-IgDownload protocol
IHC protocol for FXYD6 antibody 68058-1-IgDownload protocol
WB protocol for FXYD6 antibody 68058-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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