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FILIP1L Polyclonal antibody

FILIP1L Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 30134-1-AP

Print datasheet

Synonyms

COL4A3BPIP, DOC 1, DOC1, FILIP1L, GIP90

验证数据展示

  • WB analysis using 30134-1-AP

    WB analysis using 30134-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30134-1-AP (FILIP1L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 30134-1-AP

    IHC staining of mouse ovary using 30134-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 30134-1-AP (FILIP1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse ovary using 30134-1-AP

    IHC staining of mouse ovary using 30134-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 30134-1-AP (FILIP1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNIH/3T3 cells, mouse colon tissue
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30134-1-AP targets FILIP1L in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen FILIP1L fusion protein Ag32655 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name filamin A interacting protein 1-like
Synonyms COL4A3BPIP, DOC 1, DOC1, FILIP1L, GIP90
Calculated Molecular Weight 893 aa, 102 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC027860
Gene Symbol FILIP1L
Gene ID (NCBI) 11259
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4L180
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FILIP1L antibody 30134-1-APDownload protocol
IHC protocol for FILIP1L antibody 30134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols