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验证数据展示

  • FC experiment of HeLa using CL555-20618

    FC experiment of HeLa using CL555-20618

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®555-conjugated FAM83A Polyclonal antibody (CL555-20618)(red), or 0.8 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated FAM83A Polyclonal antibody

FAM83A Polyclonal Antibody for FC (Intra), ELISA
Cat No. CL555-20618

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), ELISA

别名

BJ TSA 9, Protein FAM83A, TSGP, Tumor antigen BJ TSA 9, Tumor antigen BJ-TSA-9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 20618-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.8 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-20618 targets FAM83A in FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 FAM83A fusion protein Ag14680 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 83, member A
别名 BJ TSA 9, Protein FAM83A, TSGP, Tumor antigen BJ TSA 9, Tumor antigen BJ-TSA-9
计算分子量 434 aa, 47 kDa
观测分子量 47-58 kDa
GenBank蛋白编号BC007828
基因名称 FAM83A
Gene ID (NCBI) 84985
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UY5
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL555 FAM83A antibody CL555-20618Download protocol
Standard Protocols
Click here to view our Standard Protocols