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验证数据展示

  • WB analysis using 26569-1-AP

    WB analysis using 26569-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26569-1-AP (FAM54A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 26569-1-AP

    IF Staining of HeLa using 26569-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FAM54A antibody (26569-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

FAM54A Polyclonal antibody

FAM54A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26569-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

MTFR2, Mitochondrial fission regulator 2, DUFD1, DUF729 domain-containing protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, Kasumi-1 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26569-1-AP targets FAM54A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23711

Product name: Recombinant human FAM54A protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-300 aa of BC063688

Sequence: MSLILNILREMLEYFGVPVEQVLLIWENKDYGSTRSIVRIIGKMLPLEPCRRPNFELIPLLNSVDSDNCGSMVPSFADILYVANDEEASYLRFRNSIWKNEEEKVEIFHPLRLVRDPLSPAVRQKETVKNDLPVNEAAIRKIAALENELTFLRSQIAAIVEMQELKNSTNSSSFGLSDERISLGQLSSSRAAHLSVDPDQLPGSVLSPPPPPPLPPQFSSLQPPCFPPVQPGSNNICDSDNPATEMSKQNPAANKTNYSHHSKSQRNKDIPNMLDVLKDMNKVKLRAIERSPGGRPIHKR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 54, member A
别名 MTFR2, Mitochondrial fission regulator 2, DUFD1, DUF729 domain-containing protein 1
观测分子量43 kDa
GenBank蛋白编号BC063688
基因名称 FAM54A
Gene ID (NCBI) 113115
RRIDAB_3085885
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P444
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Family with sequence similarity 54 member A (FAM54A), also known as MTFR2 or DUFD1, is a mitochondrial outer membrane protein that controls embryonic development and cell differentiation. FAM54A is co-transcribed with core centromere/kinetochore components and plays a critical role in bridging proper mitochondrial fission and chromosome segregation.

实验方案

Product Specific Protocols
IF protocol for FAM54A antibody 26569-1-APDownload protocol
WB protocol for FAM54A antibody 26569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Adv Sci (Weinh)

MTFR2-Mediated Fission Drives Fatty Acid and Mitochondrial Co-Transfer from Hepatic Stellate Cells to Tumor Cells Fueling Oncogenesis

Authors - La Zhang
View Article
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